Position of an Active Thiosulfonate Group in New Phenolic Antioxidants is Critical for ARE-Mediated Induction of GSTP1 and NQO1
نویسنده
چکیده
Synthetic water-soluble phenolic antioxidants are known anti-inflammatory chemicals, some of them were shown to induce components of antioxidative defense enzymes NADPH:quinone oxidoreductase (NQO1) and glutathione-S-transferase (GST) via activation of Nrf2 pathway. Our experimental in vivo models have demonstrated an increase of both gstp1 and nqo1 expression in the liver of mice treated with novel organosulfur phenolic compounds TS-12, TS-13, TS-14. Both expression and activity of GSTP1 was dependent on the distance of active thiosulfonate group in paraposition from the benzene ring of the above antioxidants. The increase of nqo1 and gstp1 gene expression was mediated by the antioxidant-binding response element (ARE). Our results revealed the role of Nrf2/ARE-dependent transcription regulation in response to the new phenolic antioxidants and suggested possible strategy to improve antioxidative properties of synthetic phenolic compounds.
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